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Distortion was also observed in samples that contains lipids, especially in the Hoki roe homogenate. Pokhariyal et al. [49] reported the complexity of protein bands during the SDS-Webpage was enhanced from the existence of lipid, which triggered distortion of protein bands.
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This supports the competition that these pretreatments have an impact on the protein framework and substrate atmosphere, thus minimizing hydrolysis more than when only one pretreatment is applied.
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